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Dr Siegmund has found that the removal of background noise and adjustment for dye bias removes the commonly observed trend of increasing signals across an array.
One unit of enzyme activity was defined as the amount of enzyme catalyzing the oxidation of 1 μmol NADH per minute after removal of background absorption.
All our analyses involved the manual removal of background fluorescence using the MxPro - MX3000P v4.10 (Stratagene) after each run.
In each experiment, data acquired from five single transfected and double transfected cells were analyzed for FRET efficiency after removal of background and spectral bleed-through by using the pFRET software [31].
In contrast the covalent capture of thiophosphorylated peptides allowed more stringent removal of background peptides, which is reflected in the data obtained by this approach.
In the present study, we have investigated the use of ox bile and micrococcal nuclease for selective lysis of blood cells and removal of background human DNA.
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All Illumina array scans were initially processed using Illumina's BeadStudio software using default parameters, including removal of local background signal in the captured images but without background normalisation.
Usually, parameter manipulating of the background subtraction method is necessary for the efficient removal of the background, which makes the quality of the spectrum empirically dependent.
For example, cortical cooling of the secondary somatosensory cortex can reduce the background activity in SI neurons, an effect thought to be mediated by removal of a background facilitatory influence [53].
b Processed image after conversion to gray scale for removal of the background.
Apart from removal of the background signal, 82Rb and 15O-water data were processed analogously.
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