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DNA was removed with RapidOut DNA Removal Kit (Thermo Scientific).
Before reverse transcription, ribosomal RNA was removed using Ribo-Zero rRNA removal kit (Bacteria; Illumina, USA).
After lysis, SDS was removed using Detergent-OUT SDS removal kit (Merck, Darmstadt, Germany).
Labeling was performed at 37°C for 1 3 h and the unincorporated nucleotides were removed using the QiaQuick Nucleotide Removal kit.
Unincorporated radioactive nucleotides were removed using the QIAquick Nucleotide Removal kit (Qiagen).
Dead cells were removed using a dead cell removal kit (Miltenyi Biotech).
Unincorporated dye was removed using a QIAquick Nucleotide Removal Kit (QIAGEN) as specified in the manufacturer's protocol.
Ribosomal RNAs were removed using the Ribo-Zero™ rRNA Removal Kit for Gram-Positive Bacteria (EPICENTRE Biotechnologies, Madison, WI).
rRNA was removed using the Ribo-Zero rRNA Removal Kit (Epicentre), and the remaining RNA was purified using the RNeasy MinElute Cleanup Kit (Qiagen).
Probes were synthesized and removed using the StripAble RNA probe synthesis and removal kit (Ambion).
Excess 20 bp oligonucleotides were removed by passage through a Qiagen QIAquick nucleotide removal kit, according to manufacturer's instructions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com