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The chromatograms do not show the characteristic peaks that can be attributed to the hydrogels used, thus confirming the absence of hydrogel residues on the paper samples after gel removal (data not shown).
Non-optimal residues such as charged or hydrophilic in nature, required larger (at least 10 times) amounts of ERAP1 for their removal (data not shown).
Priming of PC12 cells with hNGF or hNGFR100E for one week equally induced NGF dependency upon neurotrophin removal (data not shown), and survival and differentiation of primed rat PC12 cells, induced by 50 ng/ml hNGF and hNGFR100E addition after replating, was identical, both in terms of number of surviving and differentiating cells and of time course and extent of neurite outgrowth (Fig. 2D F).
Blocking DR6 by 5D10 also promoted rat motor neuron survival following growth factor removal (data not shown).
One clone, showing a fourfold induction in response to tetracycline removal (data not shown), was used for subsequent gene trapping.
However, by neither of these approaches were we able to demonstrate consistently strong effects on Ku removal (data not shown).
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A screening process was performed in order to select the metal oxide having the high Cd II) removal capacity (data not shown), and magnesium oxide (MgO) was selected as high Cd II) up taking agent among the metal oxides tested.
The tdT fluorescence can be detected after a single day of Dox removal using immunohistochemistry (data not shown), and weak tdT fluorescence can be detected without immunostaining 3 days after Dox removal (Figure 2B).
Similar differences existed when comparing protocol G vs. protocol H, which only differed by the removal of TOF (data not shown).
The cell-surface expression of HLA-A and -B was decreased to the levels of the non-induced conditions 72 h after removal of IFNγ (data not shown).
Expression of mature DC surface markers remained stable for 48 hours after removal of cytokines (data not shown).
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