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Exact(21)
Subsequently, any unincorporated labeled dNTP's were removed using Dye-X removal column purification kit (Qiagen).
cSPLUNC1 was suspended in 200 µl water and residual SDS was removed using a detergent removal column (Millipore, Billerica, MA).
For 2D gel electrophoresis, albumin was removed using an albumin removal column (Calbiochem, San Diego, CA) and samples were concentrated using Centricon YM-10 spin column with protein concentrations determined by Bradford assay (Bio-Rad Laboratories, Hempstead, United Kingdomingdom).
We also designed a d-limonene removal column (LRC) that successfully removed this inhibitor from the fruit waste.
The inhibitor was removed from DEAEA by passing the monomer two times over an inhibitor removal column from Sigma (Castle Hill NSW, Australia).
Open image in new window Fig. 5 Vertical load versus downward displacement of removal column.
Similar(39)
Unincorporated nucleotides and random primers were removed using QIAquick Nucleotide Removal columns (QIAGEN) according to the manufacturer's instructions.
Ricin sulf-1 was fluorescently labeled with Cy2 bis-Reactive Dye Pack (GE Healthcare Biosciences, Uppsala, Sweden), and purified with Fluorescent Dye Removal Columns purchased from Thermo Scientific (Rockford, Il, USA), according to the manufacturer's procedure.
Subsequent purification applying EndoTrap removal columns (Hyglos, Bernried, Germany) yielded a reduction to approx. 1.1 EU/mg S100A1 protein.
The protein was taken through an endotoxin removal process using detoxigel endotoxin removal columns (ThermoFisher Scientific, Waltham, MA) following manufacturer's protocol.
Total RNA was extracted using the RNeasy Plus Mini Kit, Qiashredder columns and gDNA removal columns (Qiagen, Valencia, CA, USA) according to standard supplier protocols.
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