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This dissociation is observed for polyadenylated targets undergoing deadenylation and may represent a remodelling step for mRNAs entering the 5′-to-3′ degradation pathway.
This clathrin-mediated remodelling step is a common feature in neuroendocrine and endocrine secretory granules, and is thought to provide a mechanism for proof-reading the content and membrane composition of the maturing ISG to ensure the production of MSGs which contain biologically active hormones and can undergo efficient exocytosis.
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This indicated that most nucleosomes had indeed being driven to slide on the DNA during the initial remodeling step.
Our analysis of ∼200 molecules indicated that 23±3 of the 31±4 nucleosomes initially present on the plasmids remained bound after remodeling step, with the remaining nucleosomes ejected.
Furthermore, in the insert orientation with the 5 extra nucleotides positioned 50 bp from the 601 dyad (Figure 8B, blue arrow), the fifth remodeling step was larger for the 94 bp insert than for the 89 bp insert.
Osteoclast at the end of remodeling step undergoes apoptosis via phagocytosis [ 4– 8].
BTHS is caused by mutations in the gene encoding tafazzin, the transacylase that carries out the second CL remodeling step.
A remodeling step makes sure that the predetermined spot boundaries from the consensus are adapted to the real gray levels observed on the target image.
The spot sizes and intensities differ from gel to gel, so a spot remodeling step is applied to make the spot boundaries fit to the gray level distributions of the original gel images.
Upon weaning of the progeny, the mammary gland undergoes an important remodeling step, termed involution, during which the unessential mammary epithelial cells (MECs) die and are largely removed by non-professional phagocytes, that is, the epithelium itself.
Perhaps, therefore, a specific post-translational modification of H3.3 might be associated with HIRA-dependent deposition, or an additional chromatin remodeling step at the point of H3.3 deposition might promote an active chromatin conformation and accessibility to the underlying DNA sequence.
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