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As can be seen from Figure 1, the essential improvement of the new tip functionalization method rests on the use of a masked aldehyde (i.e., an acetal) which remains unreactive while amide bonds are formed between the linker and the amino groups on the tip surface.
The process of bisulfite treatment exploits the different sensitivity of cytosine and 5-MeC to deamination by bisulfite under acidic conditions, in which cytosine undergoes conversion to uracil while 5-MeC remains unreactive.
The process of bisulphite treatment exploits the different sensitivities of cytosine and 5-methylcytosine to deamination by bisulphite under acidic conditions, in which cytosine undergoes conversion to uracil, whereas 5-methylcytosine remains unreactive [ 55].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com