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Tissue samples from four of the six chimpanzee individuals were used in both protein and mRNA expression analysis, while the remaining tissue samples used for protein and mRNA expression analysis did not overlap.
No infectious virus or viral nucleic acid was detected in any of the remaining tissue samples from any of the virus challenge pigs or from any of the negative control pigs.
For an overview of the principal tissue or scaffold organization, histologic sections were obtained from selected remaining tissue samples or scaffold material.
The remaining tissue samples were immediately stored at −80 °C until use.
The remaining tissue samples were included in the control CE group.
Remaining tissue samples were provided between two and six sites surrounding the implant.
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In case of a completely cartilaginous construct (such as the pellets we used) the remaining tissue is cartilage and the sample harvested after 14 weeks demonstrated that the complete construct is subsequently turned into bone and bone marrow.
Subcutaneous fat and deep dermis were excised from the sample, and the remaining tissue was cut into smaller pieces, followed by trypsinization (0.25% trypsin, Gibco) at 37°C for 30 min.
The remaining tissue was stored permanently in 10% formalin to allow for additional tissue sampling.
A portion was fixed and processed for histology, and the remaining tissue was dissociated to single cell and assayed following the same protocol adopted for the primary samples.
Of the remaining 133 tissue samples, 129 could be conclusively diagnosed.
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