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The Citizens United decision eliminated the biggest remaining restriction by allowing independent groups to pay for campaign ads that explicitly endorsed or opposed a candidate.
Women became able to apply for all positions other than special forces roles in the Army on 1 January 2013; it is planned that this remaining restriction will be removed in 2014 once the physical fitness standards required for service in these units are determined.
The remaining restriction enzyme molecules cleave the unmethylated recognition sequence and cause cell death.
Indeed, upon selection ∼1% of the females were weakly fertile, giving a few escaper F1 progeny harboring a variety of mutant alleles that inactivate the remaining restriction site.
Prior to 454-pyrosequencing, unique MID-barcodes were added to the remaining restriction fragments for each individual sample via polymerase chain reaction (PCR).
The only remaining restriction is that shops must stay closed on all but a few Sundays a year.
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They still bristle at the remaining restrictions.
Since then, Beijing has relaxed some of its remaining restrictions on visits here by mainland citizens.
The new British-American plan would have removed remaining restrictions on civilian trade.
Then, last month, a court directed the government to lift remaining restrictions.
More may come in May when the remaining restrictions lapse and a good thing too.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com