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We observed a similar abnormality in 50% of islet preparations from T2D organ donors; the remaining preparations exhibited normal glucose regulation.
The remaining preparations generated purely tonic spike activity, most likely because they did not express locomotor activity during the recording period (see below).
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The final mitochondrial suspension was immediately used for measurement of mitochondrial respiratory parameters, and the remaining preparation later stored at −80°C for production of protein lysates for immunoblotting.
The remaining two preparations were used for qPCR validation of microarray results.
In the remaining five preparations, glucose inhibited glucagon secretion by 44% ± 9% (data not shown).
In the remaining four preparations, C2 stimulation caused only depolarization with multiple components (cf., Figure 6B iv, Animal 4).
One-third of the concentrated early-morning sputum pellet remaining after preparation of the microscopy slides and culture slants was frozen and shipped to the National Institutes of Health NIH Clinical Centerer, Bethesda, MD.
Blood remaining after preparation of blood smears was added to an EDTA tube and frozen (−80°C) until processed.
However, given that parts of the girdles and vertebral column were protruding from the remaining steinkern, preparation was initiated in recent years resulting in the exposure of the girdles and portions of the vertebral column associated with the shell.
Briefly, a 4 ml-aliquot of residual PreservCyt remaining after preparation of the thin-layer slide was used for HC2 testing and estimation of viral load when a single, targeted HPV type was present.
Insoluble material remaining after preparation of whole-cell extract (WCE) was reextracted in high-salt (HS) solution (400 mM NaCl) buffer as described previously [ 32], except that the deubiquitinase inhibitor N-ethylmaleimide (NEM) was added to a final concentration of 0.5 μM.
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