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Many of the organisms usually categorized as protists are placed in one of the other four kingdoms; protoctists make up the remaining organisms, which are all aquatic, and include algae and slime molds.
Ten individuals were fed with the food source DC, and the remaining organisms were fed with leaf discs type DS.
The cinderblocks were then soaked in a dilute chlorine bleach solution for ∼30 min, scrubbed with a brush to remove remaining organisms, and then soaked in fresh water.
If oxygen-requiring organisms perish, the remaining organisms will be air-breathing insects and anaerobic (not requiring oxygen) bacteria [ 40].
Thus, for the remaining organisms, we considered only genes of known or predicted function, conserved genes, and genes of unknown function.
The eight remaining organisms (including two bacteria and six archaea), were analyzed using MetaGeneAnnotator [ 57] to identify genes and MetaTISA [ 22] to locate Translation Initiation Sites (TISs).
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Next, remaining organism-antimicrobial pairs were categorized as possessing no intrinsic activity (NA), no VA susceptibility data available (ND), or further confirmation of susceptibility estimates required.
We labeled 13 organisms as thermophiles, and the remaining 36 organisms as nonthermophiles.
Only 167 of the initial 200 organisms were included in the exported clusters; 29 of the 33 lacking organisms contained zero or only one GO term, and the remaining four organisms had a unique combination of two GO terms that were not found in other organisms, thus these organisms could not be clustered.
We excluded from the analyses organisms that reassign stop codons, and for the remaining 1,976 organisms we removed incomplete or ambiguous transcripts.
The room underwent a four-hour ozone shock treatment to kill any remaining living organisms and zap associated odors.
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