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Upon addition of purified HOPS, Sec17p, Sec18p, and Mg2+ ATP, fusion allows the biotin-R-phycoerythrin to bind to the Cy5-streptavidin within the lumen of fused vesicles while remaining inaccessible to the external, nonfluorescent streptavidin.
Spread over 45 110 km, sandwiched between Karakoram in the north and Trans-Himalaya in the south and 80% comprising of a rural population with many villages high up in the mountains remaining inaccessible during winter, logistics for conducting a comprehensive epidemiological study representative of the whole population is formidable.
It would in principle be ideal to probe as much of the binding landscape as possible, but it is crucial to realize that most experimental methods query nonoverlapping regions of this field, with many regions remaining inaccessible due to experimental constraints.
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The report, however, notes that 90percentt remain inaccessible.
Although Random House has made inroads in many major Western markets and in Australia, Japan has remained inaccessible.
Facebook reports access problems and will remain inaccessible from China as of January 2010.
The Justice Department's site and several others remained inaccessible for much of Thursday afternoon.
Whole areas of Caravaggio's life and work remain inaccessible today to even the most meticulous scholarship.
Even though your novels are mostly set in Pakistan, they remain inaccessible to the majority of the people therein.
Remarkably, many files about nationalists, marked "confidential" by the British, remained inaccessible in the post-independence period.
Remote areas near Sikkim's northern border with Tibet remained inaccessible on Wednesday as rain washed away roads.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com