Six additional sets of models as those described above but each with speed centered at one of the three remaining calibration-trial walking speeds (4.5, 5.5, and 6.5 km · h−1) and with different calibration-method reference categories (treadmill vs. track) were constructed.
If a calibration is accurate, provided the remaining calibrations are reliable and the data and model are appropriate, the posterior estimate should remain within the prior range even in the absence of the fossil constraint.
The remaining four calibration points are deemed less reliable because they are based on phylogenetic analyses of morphological data only.
Moreover, the calibrations were reciprocally compatible: the remaining five calibrations always recovered a posterior estimate (± SD) for the excluded node within the time window independently obtained from the corresponding fossil evidence (Additional files 1 and 2).
The remaining fossil calibrations mark nodes within the campanulids.
With the exact algorithm being proprietary, it can be assumed that TPTD-derived calibration has impact on a "patient-specific calibration-factor" remaining constant until the next calibration ("cal"; Figure 7).
That is, type B uncertainties that may affect the accuracy of the calibration include remaining errors due to non-linearities of the x-ray images.
We then obtained an updated ADO index in half of our cohorts to improve its predictive accuracy, which in turn was validated comprehensively in the remaining cohorts using discrimination, calibration and decision curve analysis and a number of sensitivity analyses.
On the basis of expert opinion and model calibration, the remaining tests are performed: (1) following an NT>3.5 mm (n=398), (2) for other indications (but samples are also tested for T21; n=1814) and (3) in pregnant women who want more certainty without being at increased risk (n=1000).
