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In all the strains under study, significant amounts of glucose (8.0-16.5 g L-1) remained unconsumed in the media even after 96 h of incubation.
Cis,cis-muconate and catechol were excreted during growth on benzoate, but while catechol was subsequently taken up, cis,cis-muconate remained unconsumed (Fig. 2c).
4-hydroxybenzoate from p-coumarate, catechol from benzoate, vanillyl alcohol and vanillate from vanillin were subsequently consumed, except for cis,cis-muconate (from benzoate), which remained unconsumed even after 20 h (Fig. 2).
In the steady state, when 0 < D < 1 2 D W essentially all the nutrient was consumed by the culture as fast as the nutrient entered the chemostat but when 1 2 D W < D < D W significant concentrations of nutrient remained unconsumed.
Similar results showed that furfural was completely consumed by the yeast C. curvatus but HMF remained unconsumed during fermentation [ 22].
When the control was supplemented with β-glucosidase, the amount of cellulose that remained unconsumed (25 g/L) was similar to that of cultures of the ∆ pox strain expressing β-glucosidases after 5 days of growth.
Similar(54)
At Le<1, prior to the total extinction, part of the deficient reactant escapes the reaction zone and remains unconsumed.
Authors observed that, at lower C/N ratio such as 5, 40% unconsumed NH4+-N remained in the reactor due to dearth of the carbonaceous substrate.
In addition, there were only low levels of unconsumed sugar remaining in the broth at the end of fermentation, indicating that both fermentation and hydrolysis were negatively impacted for the two highly down-regulated COMT feedstock lines.
Risk remained.
None remained.
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