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It also emphasized that although ORF75c− mutants were attenuated, they remained replication competent.
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Both ΔORF2-PRRSV and ΔORF4-PRRSV viruses were propagated for 40 50 generations in the corresponding complementing cells and remained replication-defective in MARC-145 cells.
By contrast, it remains debated whether RNA viruses or retroviruses can encode and express viral miRNAs and remain replication competent.
This also ensured that the smallest significant remaining replication time was still larger than the largest error which we can expect due to the smoothening of the profiles.
The remaining
In a second step we identified all segments whose remaining replication time (deviation from the approximated segment-dependent replication time) was significantly different from the prior normal distribution on a significance level of 0.05 with the Holm-Bonferroni correction applied [39].
With the exception of 2B and 3A, the remaining replication proteins of RV89 (2C, 2C, 3C, 3D) could be either expressed and purified in E. coli or produced as synthetic peptide (3B) (Supplemental Table 1, Supplemental Fig. 1).
The remaining five replication origins were either present or absent in an individual strain, that is, the distribution of these origins was variable in Haloarcula species (table 1).
In contrast, we found that the nucleoside analog Ribavirin, which inhibits RNA replication, remained inhibitory when added one hour post-infection (Figure 4).
For A3, the data could be explained if replication remained efficient but Sup35-GFP was incorporated into prion polymers at a faster rate, which would confer a stronger phenotype.
If it is too large, after a copy reaches the destination, there still remain many replications and possess the storage resources.
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