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In other words, we wanted to see if low remained low, medium remained medium and high remained high.
When examining the effect of all stressor categories on muscle activity stratified to body region (i.e. neck shoulder muscles and forearm muscles), the effect remained medium; Hedges' g = 0.39 (p < 0.01) and 0.33 (p < 0.01) for the neck shoulder and forearm muscles, respectively (Fig. 3).
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Even so, a large gap of unexplored territory was obviously still left in the remaining medium to high vA range.
Before the reagent was added, 3.0 mL of cultured medium was transferred to other dishes at 37 °C, and the membrane was transferred to each medium in turn (1 mL reagent medium for 30 min; 1 mL wash medium for 10 min; and 1 mL remaining medium for monitoring of bioluminescence).
"Mobile is the last remaining medium where the user has to bear the entire cost of the content," he said, "and that risks stunting the growth of many new and innovative mobile services that clients might be willing to access if the costs were lower".
In order to validate the adequacy of the model, the confirmation experiment was carried out using medium prepared according to the RSM results (the remaining medium components at the following concentrations g/L: MgCO3 3.0, CaCl2 0.5, K2HPO4 1.0 and NaCl 1.0).
The receptor medium within each donor chamber was removed and the surface of the skin was gently wiped with a cotton swab in a standardized way to remove the remaining medium.
Concentration of remaining medium ingredients and culture parameters was taken from the performed Plackett Burman model, depending on the positive or negative effect of either tested low (− 1) or high (+ 1) value.
Although homeopaths maintain that the ingredient, although no longer present, has left its impression on the remaining medium, the committee is, of course, skeptical.
The remaining medium in the upper part was taken and checked for Paramecium.
The remaining medium was immediately replaced with fresh medium and cytotoxicity was determined by MTT assay (see Cell vitality).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com