In contrast, a higher concentration was used in this exploratory phase of recombinant S. albidoflavus chitinases since only an affinity chromatograpy purification protocol was applied to the protein extracts and the protein still remained bound to MBP.
This confirms that streptavidin remained bound to DNA throughout the reactions, consistent with previous reports that WRN helicase does not dissociate the streptavidin from the DNA [49].
As evident from the FRET histograms, most of RAD52 or RAD52Y104pCMF remained bound to DNA after washing.
After digestion, the 5' cDNA ends were washed away while the 3' cDNA fragments remained bound to Oligo (dT) beads.
Uncleaved fusion protein, His6-TEV, and His6-maltose-binding protein remained bound to the column and were eluted with a TNG/250 mM imidazole wash step.
When chondrocytes were treated with both TNF-α and atRA, the complex that remained bound to DNA contained p65 and RARα.
Upon binding of RAD52, FRET increased and continuously fluctuated between intermediate and high FRET states, while the RAD52 oligomer remained bound to DNA.
The tissue fragments remained bound to the slides throughout the subsequent FISH processing steps and produced good quality FISH chromosomal signals.
The result is shown in Figure 6A, whereby VP2-GFP was found to be slightly dissociation at 0.3 M NaCl and 0.5 M NaCl, but a significant amount of remaining protein remained bound to the chromatin.
In all of his work, his versatility remained bound to the social outlook that characterized the "civic Humanism" of Florence.
After many of its colonies won independence in 1960, many remained bound to France, using a currency pegged to the franc and then the euro, and maintaining close trade, military and diplomatic ties.
