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In this way, only the uPAR-bound internalized antibodies remain labeled.
It is usually recognized that most commercial products remain labeled in international units.
Additionally, we previously established that LRECs are in cycle, as demonstrated by the labeling of DNA with a second label (5BrdU) that rapidly disperses to daughter cells [ 4], proving that LRECs are dividing asymmetrically as they remain labeled with [H]thymidine.
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Top panel on the left shows the remaining labeled mtRNAs.
Right panel shows the quantification of the remaining labeled mtRNAs normalized to Mortalin signal (n = 3).
Control and treated zygotes remained labeled throughout the duration of the experiment.
2. By then manually subtracting the remaining labeled objects that by morphological criteria and comparison to the original image seemed to result from nuclear labeling.
TCEP treatment led to the rapid (within seconds) and almost quantitative removal of cell surface fluorescence such that only receptors within endosomal compartments remained labeled.
At the onset of puberty at three weeks of age, 8.6% of epithelial cells remained labeled but by eight weeks, only 1.1% of epithelial cells were EdU-positive.
Non-lens ectoderm remained labeled at 24 hpf (Fig. 7P), indicating that the prospective lens cells at 12 hpf are intermixed with non-lens cells.
In both cases, the remaining labeled cells could not be positively identified as Müller cells, based on either their position within the nuclear layer or by labeling with the Müller cell marker.
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