Some base-callers explicitly model the biases, while other rely on reference sets to train general purpose classifiers; as we discuss in the main text, both approaches have their pros and cons.
They also raise the possibility of conflicts of interest, because the rules rely on reference guides that in some cases are linked to drug makers.
That exercise, like the overturned chair, taught crew members to choose an exit and then rely on "reference points" to get there — firm handholds inside the aircraft with which to pull themselves, handhold by handhold, toward an opening.
Alignment-based transcriptome assembly methods, which rely on reference genomes and additional annotation information, may suffer from missing/erroneous information.
Of course, such work would also rely on reference standards of these metabolites, many of which are not currently obtainable.
This program was chosen over other programs as it does not rely on reference genomes, of which there are none for the Phasmatodea.
However, similarity-based methods rely on reference databases that contain sequences of known genomes, so these methods cannot classify the majority of sequences that were from unknown genomes without close references.
Studies that cannot rely on reference genomes will, therefore, not be hampered by a lack of a priori information on linkage information when building maps and will most likely perform better when using the AUTOGROUP method.
Transcriptome de novo assembly was carried out with Trinity software [ 78] that can recover full-length transcripts across a broad range of expression levels, with sensitivity similar to methods that rely on reference genome alignments.
Calibration protocols that do not rely on reference-quality PG measurements must be explored for use in the outpatient environment and sensors that perform better in tracking PG would be expected to improve mean PG and reduce hypoglycemia.
