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Moreover, the interaction between MALAT1 and methylated or unmethylated Pc2 controls the relocation of cell cycle related genes in PcG bodies or interchromatin granules (ICG), resulting in gene repression or activation [ 82] (Table 1).
The actin filaments were localized at the level of the aposomal constriction since they are associated with the cell adhesion belt [ 46]) and are also indicators for distal relocation of cell junctions [ 37].
To quantify the void space created within cell walls by relocation of cell wall matrix material and nanofibrillation of cellulose, TEM images were processed to threshold intra-wall void spaces into regions from which size and shape descriptors could be calculated.
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The method facilitates easier sample handling and relocation of cells in embedded blocks.
Gridded dishes allowed relocation of cells with activated chromosomes.
This method, termed MAINTRAC® analysis, enables relocation of cells for visual examination and quantification, and for taking fluoromicrographs.
Zabaglo et al. modified the cell filtration technique with laser scanning cytometry (LSC), allowing for the relocation of cells expressing cytokeratin for further analysis.
Although MYC activation is known to promote IFE thickening and to cause hair follicle abnormalities in K14MycER mice (Arnold and Watt, 2001), lineage tracing established that there was no relocation of cells from the IFE to the SG (data not shown).
Cells were cultivated and fixed as above except that grided coverslips (Belco Biotechnology, Etched GRID coverslip 23 × 23 mm, stock No. 1916-92525, distribytElectron Microscopy Sciencesiences, Ft. Washington, PA, (#72264-23) and obtained through Science Services, Munich, Germany (#141204)) were used to allow relocation of cells.
In fact, we regard automated relocation of carcinoma cells identified in Papanicolaou-stained specimens as a prerequisite of FISH analysis in cases in which cancer cells are admixed with a high proportion of benign cells.
Recent studies suggest that the formation of pathological microenvironments featuring substantial relocation of hematopoietic cells within a peripheral organ can impact other distal organs besides the BM, where hematopoiesis takes place.
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