To test whether the Frz system promotes RomR relocation by inducing phosphorylation of RomR, we introduced plasmids encoding RomRD53N-GFP and RomRD53E-GFP into a romR:: nptII mutant containing the frz mutation.
Non-genomic effects include an Akt-mediated stimulation of glucose induced relocation of glucose transporter type 2 (GLUT2) in β-cells.
To further confirm that heat induced Rom2 relocation is caused by Mpk1 activation, we overexpressed MKK1S386P in both wild type and mpk1 deletion cells.
Western blot analysis showed that treatment of MDA-MB-231-ER:FOXO3a(A3) cells with 4-OHT induced the relocation of FOXO3a from the cytoplasm to the nucleus fractions (Fig. 10A).
We therefore tested whether silencing of NbNAG similarly induced nuclear relocation of bZIP28 from the ER membrane.
Parkin stimulated the accumulation of impaired mitochondria around the nucleus, induced the relocation of the autophagy marker LC3 to damaged mitochondria and thus facilitated their elimination by mitophagy.
None of the PTM patterns on the actin I (see Additional file 4A-C) or the polymerase alpha nanochromosome (see Additional file 4D-F) changed during conjugation, indicating that relocation of PTMs is induced only upon gene activation.
Beyond the change of residue itself, the F241L mutation induces relocation of the K200 side chain also located within the interface between protomers of trimeric Lg-ECD, thereby explaining the defective oligomerization of mutant Lg.
The process also induces relocation of ICD bio markers including calreticulin, Hsp70 and Hsp90 to the cell surface and the rapid release of immunogenic signals including ATP and HMGB1 followed by maturation of immature dendritic cells.
Kurki et al. [ 7] showed that ultraviolet damage induces relocation of nucleophosmin from the nucleolus to the nucleoplasm, where it interacts with p53 and HDM2 and thereby stabilizes the level of p53.
