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RovA can relieve the repression of the H-NS/YmoA complex by directly competing for binding to the promoters of the regulated genes including inv [7], [8], [9].
According to this model, the destruction of Geminin when cells enter G0 phase would relieve the repression of Brg1 and other transcription proteins and trigger terminal differentiation [4], [13], [14].
Therefore we propose that the threshold level of Rot (and SarS) is required to relieve the repression imposed by SarA, and that the ClpX dependent expression of Rot is required to exceed this threshold level.
The inactivation of the four selected genes in the chromosome of E. coli strain W3110 was hypothesized to increase the cellular concentrations of pyruvate and formate, reduce the re-oxidation of evolved hydrogen, and relieve the repression of genes required for PFL and FHL biosynthesis.
Rim11 is also required to relieve the repression of Ime1.
Further, we found that Wip1 overexpression did not relieve the repression of PIK3CA, PI3KCB and IGF-1R.
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Downregulation of eIF4E2 relieved the repression, while the control RL mRNA distribution was not affected (Fig. 4B).
The mechanism by which activated YfiB relieves the repression of YfiN may be applicable to the YfiBNR system in other bacteria and to analogous outside-in signaling for c-di-GMP production, which in turn may be relevant to the development of drugs that can circumvent complicated antibiotic resistance.
Once activated by certain cell stress, the dimeric YfiB transforms from a compact conformation to a stretched conformation, allowing the periplasmic domain of the membrane-anchored YfiB to penetrate the cell wall and sequester the YfiR dimer, thus relieving the repression of YfiN.
The removal of the Aux/IAA proteins relieves the repression of auxin-responsive genes [8], [11].
Third, the epigenetic modifier 5-aza-2'-deoxycytidine relieves the repression of miR-200c/miR-141 in cancer cell lines.
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