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The minimum number of cells reliably detected above background was approximately 20,000 although some visible signal was detected at approximately 10,000 cells in at least one case (Figure 2H).
In 98 clinical specimens (78 sensitive and 20 resistant strains) no minority alleles were reliably detected above the 3% threshold of the assay.
At the 0.1% level of overall spike-in, cross-contamination down to 1% could be reliably detected above the background contamination and sample-to-sample SASI read variation, within the experiment.
Few genes across samples were detected above baseline on either platform, but a subset of probes detected above baseline levels in these samples on the DASL-based assay may show some reproducibility (Additional File 5).
When we included the four heteroplasmic concordant variants, our results suggest that 454 sequencing may detect heteroplasmy at the level reliably detected by Sanger sequencing (above ∼20%) [26] but also below that limit [14].
We limited our analysis to genes reliably detected in both replicates and with expression levels well above the background.
Shakespeare's hand has also been reliably detected in The Book of Sir Thomas More and Edward III.
A peptide was considered to be reliably detected at a q value ≤0.01.
This showed that the PersonRuns behavior could be reliably detected from this feature space.
This can be reliably detected with ultramicroscopy.
This demonstrates that blood vessel alterations can be reliably detected with our setup.
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CEO of Professional Science Editing for Scientists @ prosciediting.com