The results in this study indicate that the wireless implantable stimulator in mice reliably delivers continuous bilateral stimulation, importantly, does not restrict the animals mobility and hygiene (grooming behavior).
Thirty-five ofthef the 46 subjects who participated in Experiment 1 were able to participate in the EEG experiment; 6 reliably heard continuous tones as discontinuous, 9 had performed at chance levels with respect to their continuity perception, and 20 had reliably heard the continuous tones as continuous.
Only 61% of all participants reliably judged the continuous tone (accompanied by short higher-frequency noises) as continuous.
Sixty-one percent of the participants in the present study reliably heard a continuous tone accompanied by a brief noise burst more than one octave away as continuous, while 24% consistently judged this tone to be interrupted during the noise burst.
More detailed data collection confirmed that those participants not only reliably judged these continuous sounds as discontinuous, but also did not perceive continuity illusions at short noise durations, where previous work had shown that these illusions tended to be the strongest [5], [29].
However, in both of these time windows, nonparametric ANOVAs yielded significant main effects for subject performance (factors: condition ("noise alone" vs. "tone plus noise") and subject performance (reliably perceive as continuous vs. discontinuous); P50: H = 25.8, df = 51, p = 0.044; N270 350: H = 31.9, df = 51, p = 0.019; both corrected for multiple comparisons).
The more reliably an individual judged the continuous tone as continuous, the greater was his or her susceptibility to the continuity illusion (Figure 2b, Spearman ρ = 0.87, N = 46, p<0.001).
The system performed reliably with a mean maximum continuous recording time of 13.3 (±0.3) h.
A subject was considered to reliably perceive the sound as continuous or discontinuous if their performance differed from chance at the p<0.05 level specified by the binomial distribution (see Fig. 2a and Methods).
One of these approaches might be continuous infusion, which reliably increases levels above the breakpoint of 2 mg/l and avoids wide swings in peak and trough levels [ 7].
