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The AprilTag provides the users a large number of distinguishable codes for tags and reliable tag identification algorithm.
Thus using ChR2-YFP as a marker for PV expression, the false positive rate was only 3% (i.e. only 3% of ChR2-YFP expressing neurons did not express PV), indicating that ChR2-YFP expression provided a reliable tag for parvalbumin-expressing interneurons.
The set of tags with abundance > 1 in TT 1+2 SAGE library with reliable tag identification is listed in Additional file 1.
It should also be noted that none of the SAGE sequence tags identified in the analysis mapping to SCGB1A1 are the most reliable tag according to SAGE Genie [ 45].
The requirement for inter-annotator consensus provides a unique and reliable tag assignment to each fragment, and supports a clean training/test set, but these advantages come at a price: inter-annotator consensus on a fragment may imply that the fragment is easier to automatically classify, resulting in an easier classification task for a machine learning method.
However, even though the most reliable tag to this gene, CTTTGAGTCC did not pass statistically, the trend of reduced expression in current smokers relative to former smokers and similar expression between former and never smokers is consistent with the sequence tags that did appear in the analysis.
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Such "chain of custody" work will also mean exploring reliable tags and seals, and proliferation-proof monitoring of nuclear-storage areas.Earlier this year the defence secretary, Des Browne, suggested that representatives from the weapons labs of all five official nuclear powers meet to see if some of the technologies mastered for weapons building could in future be turned to verification tasks.
Reliable tags identification The estimated camera pose is in the tag coordinate system.
Reliable tag-to-gene mapping of NlaIII SAGE tags to UniGene clusters was obtained from SAGEmap, and each cluster was represented by the longest RefSeq gene.
For matched tags, only those observed in more than one of ten libraries were finally regarded as reliable tags.
Only the tags that had a reliable tag-to-UniGene match in SAGEmap were included for further analyses.
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