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The deletion became necessary to obtain reliable expression of the tandem constructs.
In addition, an improved gene-trap tTA could provide more reliable expression of the transactivator than a transgene construct since transcription is regulated in the context of an endogenous gene and is thus less likely to be subject to the position effect variegation that can hinder the expression of transgenes.
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Reliable expression of these QTL, including qNLB1.02 B73 and qNLB1.06 Tx303, suggests their applicability to resistance breeding.
One question, then, is whether this document represents a reliable expression of what the Pakistani security services actually believe — as opposed to what Pakistan's generals have learned that the world wants to hear from them.
A previous analysis of the tobacco transcriptome that utilized 40 k custom-designed Affymetrix microarrays reported reliable expression of 76% of the probe sets in 19 different tobacco tissues, including leaves and roots [ 19].
Here we use a synthetic biology approach to design and characterize a panel of T7 RNAP expression circuits with different modes of regulation that enable the reliable expression of downstream targets under a variety of conditions.
The optimized system resulted in reliable expression of fully assembled IgG at yields between 1 and 4 mg/L of shake flask culture for different antibodies.
This suggests that targeting transgenes to the ROSA26 locus allows repeatable and reliable expression of inserted sequences.
In the absence of drug treatment, we observed reliable expression of exogenous p19Arf driven by endogenous p53.
In light of the ubiquitous expression and the absence of silencing of genes expressed from this locus, documented both during development and following birth [30], it was felt that this approach would ensure a generalized and reliable expression of DiCre.
Unfortunately, no antibody could efficiently detect the reliable expression patterns of TβRII in the cell lines (results not shown).
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