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In doing so, two key-problems must be faced: (i) the value adopted for the "effective" period T1,which lengthens as the structure enters the non-linear range, and (ii) the reliability of the reference value adopted for Sa(T1).
These contrasting results for the two different datasets could be partly related to the reliability of the reference diagnoses for the two different datasets.
Including evidence qualifiers gives the user an idea of the reliability of the reference genome.
Correct species identification is dependent on the reliability of the reference strains and accuracy of the database used.
More is the number of microarray experiments selected, better will be the reliability of the reference genes identified by the server.
In real-time RT quantitative PCR (qPCR) the accuracy of normalized data is highly dependent on the reliability of the reference genes (RGs).
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It would be helpful to assess the reliability of these reference lines in Northeast Chinese adult population.
Reliability of the chosen reference gene, which we denominated rpl6e, was compared with two different commonly used reference genes, namely glyceraldehyde-3-phosphate dehydrogenase (gpdh) and translation elongation factor 1 alpha (tef1) with the two different algorithms of the software packages NormFinder (Andersen et al., 2004) and geNORM (Vandesompele et al., 2002).
To help account for the relative reliabilities of the references, a baseline weighting scheme is proposed.
The reliability of the best reference gene was validated by comparison between the qRT-PCR and the microarray result (Fig. 4).
Comparison between the qRT-PCR and in-house microarray data on roots demonstrated reliability of the identified reference genes to monitor the differential expression of drought-related candidate genes.
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