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In this critical condition, wire oscillation and relevant insertion errors do not allow using commercial contact or contactless sensors.
When the 5′-P group was in the downstream, template-providing end, there was relevant insertion on the opposite, labeled 3′-protruding end.
We then compared the resulting sequences to sequences flanking each of the two relevant insertion junctions in the human genome, which were determined by aligning the duplicon under study with all other duplicons.
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This outcome was not relevant, as insertion attempts were on a cadaver.
In search of strategies which could enable us to discriminate SD-associated technical artefacts from biological relevant SD insertion at domain borders, we exploited the facts that topological domains are highly conserved between mice and humans [ 36] and that the syntenic region in mice lack these large SD blocks [ 23, 27, 86].
Selection for relevant piRNA insertions then occurs at the level of individual germ cells, and in this way adaptation to the invasion of the new transposable element can occur over the lifetime of an individual [ 86].
For most loci, sequencing was neglected, but in relevant cases the insertion orthology was confirmed by sequencing, and direct repeats flanking the insertion as well as the original target site prior to transposition were traced.
This outcome was not relevant, as both insertion attempts were on a cadaver.
In addition, in the present work, we collected a relevant number of insertions from PRE-GT samples (1365 RIS and 887 RIS from PBL-GT and HSC-GT, respectively) allowing a coherent comparison of in vitro data with integration sites detected long-term in patients.
Reproducibility was mainly achieved by keeping constant the relevant parameters that influence insertion force, for example, the alignment of insertion tool and phantom.
All relevant data concerning the insertion and the maintenance of these peripheral lines were collected from the chart.
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