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This was done by manually inspecting the alignments and the nucleotide sequences of the relevant duplicates.
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The literature search identified 2436 papers, of which 2373 were discarded as being not relevant or duplicates based on the titles.
A total of 317 studies were potentially relevant after duplicate or unrelated studies were excluded.
Among these, 18 results were considered not relevant (resulting duplicated, botanical studies, or studies focusing mainly on other selected chemical compounds) and therefore excluded.
All retrieved titles and abstracts will be screened by the primary author (TLP), and relevant items duplicate screened by another author (ERM).
This special condition might also helps to explain that more genes are clinically relevant if duplicated in the X chromosome, and consequently more genes can be potentially pathogenic by duplication even when they are not tightly regulated genes, for instance by ultraconserved elements.
These additional studies were merged with our search results, following exclusion of non-relevant or duplicate citations.
Through this process, 16,549 relevant articles (excluding duplicates) were identified within the initial group.
A single reviewer examined the titles for relevant articles and duplicates were identified and removed.
The database searches yielded 7329 hits of which 3733 records were screened as potentially relevant after removing duplicates (Fig. 1).
We identified 1,477 articles, of which 270 were potentially relevant after removing duplicates and screening the title and abstract.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com