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When the obtained Hairpin 1 target DNA met Hairpin 2 labeled with glucose oxidase (GOD), the DNA cyclic amplification was activated, releasing target DNA into homogeneous solution for the next recycling.
As well as providing evidence for the Ca2+ releasing target of PLCζ, this also suggests that specific factors within the ooplasm may be required for PLCζ-mediated Ca2+ release [ 93].
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The released target DNA then hybridizes with another probe, whence the cycle starts anew.
On-resin cleavage can be induced by incubation in a Ca2+ ion-containing buffer, and the released target protein, with an extra Gly residue at its N-terminus, can then be collected.
The released target DNA could hybridize with another probe 1 probe 2 duplex to trigger the target recycling for signal amplification, while the released probe 2 hybridized with molecular beacon to restore its fluorescence for signal enhancement.
The released target DNA hybridized with another phosphate-DNA-auxiliary DNA duplex to trigger the target DNA recycling, while the released auxiliary DNA hybridized with hairpin DNA to activate DNAzyme segments.
Warren Adler recently released Target Churchill, a Cold War thriller he co-authored with Pulitzer Prize nominated Churchill biographer James C. Humes.
The released target can then hybridize with another probe to start the cycle anew.
The released target found another molecular beacon to trigger the polymerization cycle, resulting in the multiplication of the reporter Au NPs on the sensor surface.
The S- foci dissolve and release target mRNAs to allow their translation upon stimulation of the N-Methyl D-aspartate (NMDA) receptor.
The US is expected to release targets on how to increase the share of contracts going to developing country firms later this year.
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