Sentence examples for released upon addition from inspiring English sources
The resin was washed by adding 2 ml of SP wash buffer, and the purified protein was released upon addition of 250 µl SP elution buffer (20 mM Tris-HCl pH 8, 500 mM NaCl, 2 mM DTT).
The inhibition was released upon addition of 5 mM glutamate).
However, this activity is inhibited by the remainder of the protein but can be released upon addition of HOIL-1L or SHARPIN, nicely explaining why earlier studies could only detect activity in the context of the complex.
Residual H2O2 was quantified by polarographic determination of the oxygen released upon addition of 10 μg of purified beef liver catalase resuspended in 50 mM phosphate buffer pH 7.0 (Sigma) to 250 μl culture medium samples placed in an oxygen polarograph (Hansatech, Norfolk, UK).
These are incorporated in such a way that the pyrophosphate group releases upon addition of a nucleotide that results in the production of detectable light [ 61, 62].
We find that the presence of caveolae significantly increases the amount of Ca2+ released upon the addition of bradykinin in both calcium assays for cells in suspension (t test; p = 0.007) and single-cell measurements (Mann–Whitney test; p = 0.008).
The remaining 40 50% of the monellin molecules remain bound to the GroEL and are released only upon addition of ATP.
Under Ca2+-free conditions, a robust Ca2+ release upon addition of 8-Br N-cIDPR was observed.
19 Both cADPtR and cADPR (1) evoked rapid Ca2+ ion release upon addition to the permeabilized cell suspension indicating that they induce similar mechanisms of Ca2+ release.
The effects of DEX on biosynthesis were significantly reversed by the presence of RU486, though the increase in GAG release upon addition of RU486 was not statistically significant.
In addition, the Gβ2 subunits released upon activation of the heterotrimeric G protein activate specific effectors, and previous studies have shown it to be the primary beta subunit for certain Gαi signaling pathways in macrophage cells [5], [6].
