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The in vitro release was tested in fetal bovine serum preserved with 0.02% of thiomersal.
The SLH was characterized by SEM, CLSM, XRPD and DSC, and the in vitro drug release was tested using a paddle method.
The effect of the loss of the RNA binding activity of Tat on P-TEFb release was tested by titrating Tat R52/53A into reactions containing the 7SK snRNP.
The functional role of TPCs in NAADP-induced Ca2+ release was tested by suppressing TPC1 and TPC2 expression with small interfering RNA (siRNA) knockdown (assessed by RT-qPCR and western blot; Figures S3G S3I).
To confirm that the secretion defect in CAPS DKO neurons was due to lack of CAPS-1, CAPS-1 was re-introduced in CAPS DKO cells at 10 DIV via lenti-virus infection ('rescue') and DCV release was tested 4 days later.
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Other assays, e.g. based on inorganic phosphate release, were tested too, but turned out to be less suited.
Effects of BAPTA/AM on mechanically induced intracellular Ca2+ and Na+ load, as well as that of KB-R7943 on Ca2+ accumulation and glutamate release were tested using Mann Whitney U-test.
The effects of a GABA-ergic (baclofen), a dopaminergic (amphetamine) and noradrenergic (nomifensine, reboxetine and NE) pharmacologic agents on neurotransmitter release were tested using reverse microdialysis.
The effect of glutaraldehyde concentration (0.25, 0.5, 0.75, 1.0, and 1.25%) on the activity of the immobilized enzyme and rate of enzyme released was tested during a 24 h period.
Releases are tested against many Linux distributions, including RedHat Enterprise Linux, Debian, Fedora, and Ubuntu.
Differences between the effects produced by releases were tested with the Mann-Whitney test to determine whether the sequence (first or second) influenced the effect; p-values of ≤ 0.05 were considered statistically significant.
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