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Briefly, doxorubicin liposomes and DOX-loaded magnetic liposomes were placed into glass tube which contained the release solution (pH 4) and placed under HFMF for 10 min.
Clinical study was performed in 64 healthy subjects receiving a subcutaneous dose of an immediate release solution as reference formulation and an intramuscular injection of microspheres as test formulation.
After washing, microspheres were incubated in release solution, and at various time points, supernatants were collected on the days denoted in Figures 1– 6, and fresh release solution was added.
Thus, we concluded that total protein content in the release solution did not influence VEGF release rates.
Feeder free cells were passaged using EDTA-based cell release solution as in Chen et al. (2011).
In addition, GO/PPy films without drug loading underwent the same stimulation protocol as drug-loaded films, and the release solution was applied to neuron cultures.
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Key Retirement Solutions, Age Partnership and Equity Release Solutions have joined forces under the banner name Safer (Specialist Advisers for Equity Release).
DNA release solutions were purified using 10-μL ZiptipC18 pipette tips (Merck Millipore, Billerica, MA, USA).
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The cultures were incubated for 1 h at 37 °C to allow cell attachment and then were treated with release solutions from the GO/PPy-DEX films (rDEX) or a prepared DEX solution.
The DEX release solutions were obtained by applying an aggressive voltage stimulation paradigm (−2 V for 5 s, followed by 0 V for 5 s, 1000 cycles) to the GO/PPy-DEX film.
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