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This is written at the very beginning of the release, separated from the body only by a long hyphen.
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Bound ligands were released and separated from the sCD1d molecules by employing a chloroform:methanol:water extraction protocol similar to that previously described by Bligh and Dyer [25].
Cells released were separated from the tissue by filtration (100 μm mesh filters) and were pooled.
The released peptide was separated from the carrier and cleavage enzyme by size-exclusion chromatography.
"As children we can't vote, so one of the best ways for us to voice our opinion is through protest and if that's stopped or inhibited by kettling then where are we left?" His sister Rosie was released earlier and separated from her brother.
Liquid fractions containing the released sugars were separated from solid residues by centrifugation.
The released ciprofloxacin was separated from the liposome-encapsulated ciprofloxacin using the Nanosep centrifugal devices and quantified by HPLC as described previously (16).
Following the 45 min reaction of the yeast with the MESNA solution, the released proteins were separated from the yeast by centrifugation and 1 mM Bio-P1 peptide was added (synthesized by the University of Wisconsin Biotechnology Center, Sequence: NH2-CDPEK Bt DS-CONH2).
Genomic regions with homology to open reading frames (ORFs) of transposable elements (2,355) and pseudogenes (1,652) account for an additional 3,786 annotations, and (in contrast to earlier releases) are now separated from the total protein coding gene count.
The beads were separated from released products by magnetic separation, reconditioned through a repeated wash procedure with 1 × TE-buffer and, finally, prepared for the next round of purification.
Following his release from prison, Keating separated from his wife Mary.
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