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Firstly, while previously release score database [29] used nearest neighbor interpolation, we used area interpolation implemented in OpenCV [55] for more appropriate down-sampling simulation.
The ribosome release score (RRS) measures evidence of ribosome disassociation at the stop codon of a putative coding region.
Additionally, this gene presents the lowest ribosomal release score calculated, which strongly suggests that it is not being translated.
In a recent Cell paper (Guttman et al, 2013), a parameter called the Ribosome Release Score (RRS) was used to discriminate between translated protein-coding regions and non-coding transcripts with similar ribosome densities.
We have further analyzed our dataset using the ribosomal release score (RSS) metric for a number of non-coding RNAs for which we have derived TE values and compared these ncRNAs with the rest of our protein coding genes (below).
It is also important to note that select noncoding RNAs show a translational efficiency similar to coding regions, even though they are not translated (Ingolia et al., Cell, 2011, and Guttman et al., Cell, 2013); these noncoding RNAs can be distinguished from coding regions only by their ribosomal release score, which was not calculated in this study.
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