Sentence examples for release insert from inspiring English sources

and controlled internal drug release insert [CIDR, 1.38 g of progesterone (P4)]; d −3 PGF25 (PGF; 25 mg of dinoprost, i.m).; d −2 CIDR out; and AI at observed estrus (CIDR_OBS); (2) same as CIDR_OBS, but GnRH (GnRH2) 36 h after CIDR out and timed AI (TAI) 18 h later (CIDR_TAI); or (3) same as CIDR_TAI, but no CIDR (Ovsynch).

Each week, a random subset of cows in each treatment was fitted with a temperature data logger (HOBO Water Temp Pro v1, Onset Computer Co., Bourne, MA, USA) attached to a blank control internal drug release insert (Pfizer Animal Health) that was inserted into the vagina.

The cDNA clones were randomly sampled and mini-prepared for a quality check using HindIII and EcoRI double-digestion to release inserts.

The released insert was ligated into BamHI (T4 DNAP-treated)/SphI-digested pTS210 [61].

Isolated BAC clones PGB02 (3Cand and PGB04 (CYP720B4) were digested with NotI to release the insert, and insert DNA size was determined by pulse field gel electrophoresis.

Protocols which use intravaginal progesterone releasing inserts (IVP4) are effective in inducing estrus in over 90% of treated dairy cows.

One treatment used 2 intravaginal P4 implants (controlled internal drug-releasing inserts; CIDR) and the other used a GnRH treatment at beginning of the protocol.

All heifers underwent FTAI by using the "Show-Me-Synch" protocol [controlled internal drug release (CIDR) insert from d 0 to 14 followed by PGF2α (25 mg i.m). 16 d after insert removal (d 30) with GnRH (100 µg i.m). and FTAI at 66 h after PGF2α].

Estrus was synchronized in 851 heifers at four locations using the 14-d CIDR-PG protocol: controlled internal drug release (CIDR) insert (1.38 g progesterone) on Day 0, CIDR removal on Day 14, and administration of prostaglandin F2α (PG; 25 mg im) on Day 30.

Treatments were as follows: cows initiating RES 32±3 3 d after AI with no supplemental P4 (d 32 RES-CON; n = 516); same as d 32 RES-CON plus a controlled internal drug release (CIDR) insert containing P4 at the onset of Ovsynch-56 (d 32 RES-CIDR; n = 503); cows initiating RES 39 ± 3 d after AI (d 39 RES-CON; n = 494); and same as d 39 RES-CON plus a CIDR (d 39 RES-CIDR; n = 491).

The PCR product was separated on 1 % agarose (lane 2) along with DNA marker (lane 1) Fig. 2 Release of insert from pET 28a vector with restriction enzymes.