Ai Feedback
Exact(6)
Cumulative proteoglycan release (GAG in the medium) and its content in cartilages (GAG in the cartilage) were expressed as mirograms of GAG per milligram wet weight of cartilage.
A suite of laws propose to end bulk information collection, create more oversight, and release gag orders.
In vitro, cumulative GAG release, GAG and DNA content, the GAG/DNA ratio, and ∆CT values for the investigated target genes, were statistically evaluated by using the nonparametric Kruskal-Wallis test, followed by the Mann–Whitney U-test.
Previously, it was shown that enzymatic treatment of Anopheles stephensi mosquito salivary glands to release GAG disaccharides identified a HS moiety that is structurally similar to those found on liver HSPGs [ 15].
Following papain release, GAG were complexed to a final concentration of 1% with cetylpyridinium chloride (CPC), 24 hours, 26°C, and separated by centrifugation at 1400 × g for 30 minutes.
Labeled proteoglycans synthesized by cells in absence of p-nitrophenyl β-D-xylopiranoside and purified as described above, were β-eliminated to release GAG chains by alkaline digestion with 0.125 M NaOH followed by reduction with 1 M NaBH4 overnight at room temperature.
Similar(54)
Data accumulated over the past two decades shows that the interplay between these peptidases and GAGs goes both ways; cysteine cathepsins are capable of cleaving proteoglycan core proteins and thereby release GAGs from their support, whereas GAGs in turn affect both the activity and stability of cysteine cathepsins in the extracellular space.
Bovine nasal cartilage stimulated with IL-1 or retinoic acid releases GAG primarily due to aggrecanase.
By contrast, treatment of foetal bovine epiphyseal cartilage with retinoic acid, but not with IL-1, releases GAG without degrading the core protein [ 93].
Negative control tissue also released GAG in considerable amounts and this is expected in such experiment, However, it does limit the sensitivity of the system to detect differences between positive and negative control and small effects might go undetected.
However, similar to released GAG, media nitrite was significantly lower for co-treated constructs than for constructs treated with IL-1β alone up to 7 days but was not significantly different at later intervals.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com