Sentence examples for release from chromosomes from inspiring English sources

Alternatively, it is possible that in normal cells, one function of geminin is to bind and/or recruit the deSUMOylating enzymes SENP1 and SENP2 to chromosomally bound TopoIIα and to facilitate its deSUMOylation and release from chromosomes after chromosome decatenation is completed.

Cohesin release from chromosomes, as a result of its cleavage in anaphase, is essential for cohesin deacetylation, though Hos1 is present earlier (Beckouët et al. 2010; Borges et al. 2010).

In vivo, at normal concentration, geminin recruits the deSUMOylating sentrin-specific proteases SENP1 and SENP2 enzymes to deSUMOylate chromosome-bound TopoIIα and promote its release from chromosomes following completion of DNA decatenation.

These findings seem in line with the notion that the DNA tails are due to damage induced by TopoIIα's premature release from chromosomes, which occurs in geminin-overexpressing, but not geminin-silenced, cells.

To determine the timing of ring release from chromosomes, we next co-stained with MPM-2 and an antibody against separase (SEP-1), a protease that cleaves a subunit of the cohesin complex to release sister chromatid cohesion and allow homologous chromosome separation (Siomos et al., 2001; Kudo et al., 2006).

To determine the diffusion coefficient of cohesin that is not bound to chromatin, we also analyzed the FRAP redistribution kinetics of cohesin in mitotic cells, where most cohesin is released from chromosomes by the Wapl-dependent "prophase pathway" [ 16, 17, 31, 32].

To learn how geminin overexpression prematurely releases TopoIIα from chromosomes, we looked for modifications that target and/or release TopoIIα from chromosomes.

Newly released sequences from chromosomes 1 and 4 [ 33, 34] were obtained from the DNA Data Bank of Japan.

These data suggest that geminin overexpression prematurely releases TopoIIα from chromosomes before these drugs can induce binding to DNA.

Moreover, geminin and TopoIIα interact on chromosomes in G2/M/early G1 cells, and geminin overexpression prematurely releases TopoIIα from chromosomes, in part by enhancing TopoIIα deSUMOylation on chromosomes.

To validate our FLIP protocol, we compared RFI graphs of wild-type Smc3-GFP before and just after cells had undergone anaphase, when Scc1 cleavage releases cohesin from chromosomes.