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The time required to release cells from the surface was found to be longer when peptides were coupled to an extensible tether ends, suggesting that the surfaces can engender cell attachment through adhesive moieties covalently bound to the free ends of PEG chains.
One possibility is that longterm NeoA treatment, by virtue of its ability to dismantle focal adhesions and release cells from the substratum, may induce the anoikic state of apoptosis that often occurs when adherent cells are maintained in suspension [35].
Joints were homogenized and treated with collagenase to release cells from the tissue.
To release cells from the G2 phase, HeLa were first treated with 10 μM of RO-3306 for 18 h.
Alternatively, the authors could release cells from the Cdc20 arrest and allow them to enter anaphase, then score whether sister centromeres segregate to one pole or to opposite poles (see Indjeian and Murray, CurBiol 2007).
Caballe, Wenzel et al.'s findings reveal that ULK3 plays an essential role in controlling when a cell divides and imply that there may be additional proteins involved that release cells from the checkpoint delay imposed by ULK3.
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For GCs collection, the released cells from the above steps were placed in prewarmed medium of DMEM/F12.
After releasing cells from the second block, their culture medium was replaced with phenol red-free DMEM and covered with a layer of mineral oil (Sigma).
This metabolic shift releases cells from the typical restraints on growth, and provides a potential way to distinguish them from healthy cells – allowing for treatments that may be selective for cancerous cells.
The microcentrifuge tube was removed from the magnet and 1 mL of FlowComp Release Buffer (Invitrogen, USA) was added to release the cells from the beads.
Our data suggest that ALL patients might profit from therapeutic strategies that release MRD cells from the niche.
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CEO of Professional Science Editing for Scientists @ prosciediting.com