Your English writing platform
Discover LudwigExact(7)
In addition to its use for library construction and sequencing, new ultra-frequent DNA fragmentation technology based on the unique "affinity star activity" (i.e., relaxed sequence recognition) of some of the Thermus sp. family enzymes may be useful for other cloning applications.
It was demonstrated recently that the CRISPR/Cas9 system suffers from relaxed sequence specificity [ 83].
These findings suggest that the LkARS function mechanism is more reminiscent of S. cerevisiae than of S. pombe, yet bearing hallmarks of relaxed sequence selection requirements.
The large gaps remaining with this conservative mapping algorithm already shows that the reference is distant enough from the community to require a more relaxed sequence similarity search.
This pathway was already known in several studies to exhibit unusually relaxed sequence selectivity both in vivo and in vitro, but no methodical and unbiased studies have previously examined the absolute substrate selectivity of the pathway.
Interestingly, sequence chimerism in the 3' untranslated portion of the mRNA gave rise to multiple poly-A addition signals with similar strength, indicating a more relaxed sequence restriction of the 3' processing machinery than previously believed.
Similar(53)
The response to genome duplication, however, is more complex than simply relaxing sequence constraints.
Our new proposed iterative sequence (7) and relaxed iterative sequence (18) are better than the simultaneous iterative sequence of Wen et al. [18] in Example 5.1 and Zhao and Yang [17] in Example 5.2, respectively.
For the optimal read identification, we relax the sequence mismatch stringency to 10% per read, which approximates the mismatch rate allowed in Bowtie 2.
In turn, this problem can be relaxed into a sequence of convex Semi-Definite Programs (SDPs) by resorting to moments-based polynomial optimization techniques.
To determine the set of conditions leading to a fully relaxed TaqII recognition sequence and to practically apply this phenomenon to quasi-random genomic library construction, in addition to other reasons further experiments were performed at pH 8.0.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com