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WRN stimulates the ability of topo I to relax negatively supercoiled DNA and specifically stimulates the religation step of the relaxation reaction, and cell extracts from WS fibroblasts exhibit a decreased ability to unwind negatively supercoiled DNA (Laine et al 2003).
Compound 1 could arrest HeLa cell cycle G2/M and induce apoptosis by strongly diminishing the relaxation reaction of Topo II-DNA decatenation.
Compound 1 could diminish the relaxation reaction topoisomerase II DNA decatenation at a concentration of 10 μM and induce BGC-823 apoptosis by breaking DNA double-strand and activating ATM/ATR signaling pathways.
The toxin does not completely inhibit the supercoiling and relaxation reaction of gyrase but merely slows them down by about threefold (Pierrat and Maxwell 2003).
In order to find out the optimal concentration of DMSO in the EcTopo I catalyzed relaxation reaction, a DMSO dose-dependent examination was conducted during our investigations.
Conversely, an obvious promotion (Lane 6 in Figure 3(a)) was observed when 20% DMSO was added to the relaxation reaction which was originally inhibited by dsODNs.
Similar(52)
In addition to electron impact reactions, the present mechanism includes reactions involving vibrationally- and electronically-excited species, dissociative recombination reactions, three-body recombination reactions, charge transfer reactions, and relaxation reactions, taken from the literature where available, and otherwise calculated using published correlations.
Quinolones inhibit the DNA supercoiling and relaxation reactions of gyrase and can stabilise a covalent complex in which the GyrA protein is covalently attached to the 5′ ends of the broken DNA (Gellert et al. 1977; Sugino et al. 1977).
One hypothesis is that this relaxation response is a reaction to changes in the environment, part of an orienting response that is elicited by the shifts of attention caused by the repeated bilateral stimulation, which links into processes similar to what occurs during R.E.M. sleep (Stickgold 2002, 2008).
One small 2010 study of 10 healthy dog owners by researchers at Virginia Commonwealth's Center for Human-Animal Interaction found that both unfamiliar and familiar dogs provoked similar reactions: a relaxation response and reduction in blood pressure and levels of cortisol, a stress-related hormone, according to Dr. Sandra Barker, director of the center and a professor of psychiatry.
In recent years there has been a pattern of reform & repression, relaxation & reaction, thaw & freeze.
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