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The relaxation delay was 10 s.
The relaxation delay was also set to 1 s.
The relaxation delay was set to 1 s.
The acquisition time was 2 s and the relaxation delay was 3.0 s.
It has been found that increasing the relaxation delay from 5 to 20 s does significantly influences the integral value and so most of the samples were recorded with 20-s relaxation delay.
A 3 s relaxation delay was applied between FIDs.
COSY spectra were acquired with 3s relaxation delay, 6000Hz spectral width in both directions.
In the absence of proton saturation, the spectra were recorded by a relaxation delay of 5 s.
Samples were analyzed with a 5mm dual probe (35° pulse, 1-s relaxation delay,8 kilobytes of data, and 2000 scans).
A relaxation delay of 2.0 s was used, and the transfer delay was optimized for indirect coupling constants of 6 Hz.
The water signal was additionally suppressed by low-power continuous irradiation on the water resonance during the mixing time and the relaxation delay of 2.3 s.
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