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Backbone 15N relaxation data of the dimeric RBD are analyzed with the model-free (MF) method, and with the slowly relaxing local structure/molecular dynamics (SRLS-MD) approach.
The expression of a biparabolic model to represent the dielectric relaxation data of polymers is combined with a statistical method (the LEVM6 program) to provide a reasonable estimation of the parameters of this model.
In this study we have obtained low field relaxation data of protein solutions and tissue under varying conditions.
When a protein does self-associate, the observed relaxation data are a weighted average of the individual relaxation data of all species present in the association equilibrium.
Relaxation data of all samples except for rabbit papillary muscle appeared single exponential within the time resolution of the NMR experiment.
The protocol was demonstrated using the acquired 1D transverse relaxation data of nine oils, each approximately 1, 2, 3, and 4 ml, that were correlated against oil content.
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The novel model shows excellent fit of relaxation data on the canine aorta and captures the salient features of vascular viscoelasticity with significantly fewer model parameters.
The 15N-relaxation data of 2H/13C/15N-labeled Ube2g1 were measured using 800 MHz NMR at 30 °C.
The 15N-relaxation data of the amide bonds show that the acidic loop region (i.e., residues 97 109) is more flexible compared to the other structured regions of Ube2g1 Fig. 4 The summaries of the automatic assignment results from the RASPnmr analysis.
Furthermore, the model helps explain the difficulty of fitting NMR relaxation data for perturbed versions of the active-site network as a single collective exchange process (Schlegel et al., 2009).
A Master equation approach is used to derive accurate numerical relaxation data for the case of random walks on a dilute hypercube.
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