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Study limitations include the relatively limited sample size and the inability to measure IGFBP7- and TIMP-2-levels separately.
A major limitation of the study is the relatively limited sample size which impacted the power to detect the effect of certain variables deemed relevant by previous literature such as hormone/estrogen therapy, thyroid hormone, alcohol consumption and smoking (there were only 3 smokers, all men).
The main limitations of this study stem from our relatively limited sample size as well as potential biases related to our subject recruitment.
However, the relatively limited sample size in some tumor subgroups, may have limited detectable differences.
But the relatively limited sample makes state-by-state comparisons more difficult.
Anatomist Jeffrey Laitman of Mount Sinai Medical Center in New York says he finds the hypoglossal canal theory "tantalizing," but that the new study raises a cautionary note, highlighting risks of drawing conclusions from "a relatively limited sample [and] a relatively limited part of the body".
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Unfortunately, this study had relatively limited sampling of cnidarians and did not include Polypodium.
Since three of the residues illustrated in Figure 4 have only three model conformations with solvent accessibilities above 0.5 Å, the quality of the corresponding predictions suggests that even relatively limited sampling over the set of solvent-exposed conformations may often prove to be sufficient to provide reasonable estimates of amide acidity.
However, given the relatively limited phylogenetic sampling included in this study (only five taxa) the branch-sites tests may have insufficient power to detect positive selection [ 43], and more thorough phylogenetic sampling within Fundulus may increase statistical support for positive selection at these candidate loci.
With some exceptions (e.g. Lehmann et al. 2003; della Torre et al. 2005; Esnault et al. 2008; Choi and Townson 2012), the complex scenario of population subdivision within An. gambiae has been evidenced by studies that have often been based on a relatively limited geographic sampling coverage.
To date, relatively few limited sampling methodologies (four to eight samples for blood radioactivity concentration and at least one sample for metabolite analysis) have been proposed for FLT-PET [14, 19 22], and FLT kinetics has been recently estimated using image-derived input function in colorectal cancer [21], non-small-cell lung cancer [22] and high grade gliomas [23].
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