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However, in these proteins, there is more flexing of the helices of the bundle domain around the breakpoints of TMH1 and TMH6 where the relative substrates bind (Gao et al, 2010; Krishnamurthy & Gouaux, 2012; Perez et al, 2012).
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Changes in relative substrate selectivity and stereoselectivity remained however weak.
Structurally designed site-directed mutants of COMT showed altered relative substrate preferences for lignin precursors and benzaldehyde derivatives.
By nondimensionalizing the coupled-process equations for transport and nonlinear biodegradation, we show that transport behavior is controlled by three characteristic parameters: the effective maximum specific growth rate, the relative half-saturation constant, and the relative substrate-utilization coefficient.
Samples were placed above the titanium target centre and above the target racetrack in the same vertical distance to the target to examine the effect of the relative substrate to target position on the properties of the deposited coatings.
To assess the relative substrate specificity of each T. gondii enzyme, the Vmax/Km for tyrosine and phenylalanine was compared as in previous publications (27).
Aged brain γ-secretase complexes, therefore, have gender dependent substrate processing abilities with female aged brain strongly favoring APP over Notch1 as a substrate and male brain showing no significant age related changes in its relative substrate preference.
First, iRhom coexpression reduced Gurken cleavage by Rhomboid-1, expressed as relative substrate conversion.
Glucosyltransferases have relative substrate specificity because of their extended plasticity towards metabolites of related structure [ 89].
This preference, as measured by substrate utilization rate, was found to primarily depend on relative substrate availability (concentration) [ 34].
Relative substrate phosphorylation levels were determined by densitometric analysis of P signals normalized to substrate protein levels on western blots.
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