Several factors could contribute to this difference in relative regulation.
The difference in relative regulation between urg1, urg2, and urg3 seems to mainly reflect differences in basal expression levels: while all three genes show similar absolute expression signals in rich medium (containing uracil), the expression signals in minimal medium are ∼3- and 4-fold higher for urg2 and urg3, respectively, compared to urg1 based on Affymetrix chip data [19].
Relative regulation levels were derived after normalization to cyclophilin.
Relative regulation levels were derived after normalization to cyclophilin ['cyc5': ACC CCA CCG TGT TCT TCG AC; 'acyc300': CAT TTG CCA TGG ACA AGA TG (60°C, 82°C)].
The relative regulation of LTP and LTD in the different hippocampal subfields is one possible means through which the hippocampus then integrates and encodes this information into a memory engram or spatial representation.
The real-time RT-PCR results revealed the same relative regulation pattern of transcription as those of the microarray data, thereby validating and confirming the microarray results, which indicated that the expression levels of many genes were significantly changed in response to IBV infection (Table 3).
It is however interesting that in the Aubin-Horth study several genes, including beta-actin, cathepsin D, an elongation factor, beta tubulin and both vasotocin and isotocin, showed relative up-regulation in 'early migratory' fish that also showed up-regulation in our immature parr.
Exposure to clastogens, but not mutagens, resulted in a relative down-regulation of immune cell-associated genes and a relative up-regulation of fibroblast-associated genes.
Further, our data suggests that acquisition of a more invasive phenotype in melanoma requires both relative down-regulation of developmental and lineage-specific pigmentation genes partnered with up-regulation of genes likely to mediate interaction with the extracellular microenvironment of the tumour.
