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Relative quantities of intracellular pigment growing in PDB were measured using UV Vis spectroscopy.
Relative quantities of viral target cDNA were determined using REST© software.
Relative quantities of transcript levels were calculated as described by Vandesompele et al., 2002 [2002.
The SYBR green fluorescent signal was analyzed using SDS2.2.2 software, and relative quantities of GFPμ were determined.
The comparative ΔΔCT method was used to evaluate the relative quantities of each amplified product in the samples.
Relative quantities of HSV-1 DNA pol were determined from each ChIP input fraction and normalized to GADPH [23].
The relative quantities of the gene tested per sample were calculated against GAPDH using the ΔΔC(T) formula.
The relative quantities of each protein band, normalized to control cells, were quantified using Quantity One software (version 4.6.5, Bio-Rad Inc, Hercules, CA, USA).
Relative quantities of the target mRNAs were normalized against the endogenous control [by subtracting CT (threshold cycle) values for ACTB from CT values for all gene expression assays].
A standard curve was generated for each primer using dilutions of genomic DNA to calculate the relative quantities of mRNA levels for each sample.
Another study (by different investigators) examined the relative quantities of β-actin mRNA and 18S rRNA as a function of time using real-time PCR [16].
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