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Using mRNA relative quantities for 44 previously identified reporter genes taken from a large dataset comprising both CFS patients and healthy volunteers, we derived a gene profile scoring metric to accurately classify CFS and healthy samples.
The averages of the relative quantities for the biological replications (3 4) were used in a 2-tailed student t-test at a 95% confidence level (p<0.05) to determine the significance of the difference between gene expression values in female tissues versus male tissues.
The highest relative quantities for each gene are set to 1.
CT values were converted into relative quantities for analysis with geNorm [ 4].
The signal values were converted into relative quantities for analysis using geNorm.
Average values of triplicate Cq values were converted to relative quantities for NormFinder and geNorm analysis [ 21, 32].
Similar(51)
Similarly, the LNA array included 12 snoRNAs, and the TLDA contained three endogenous controls common to Cards A and B, which contributed to computing the relative quantity for each miRNA.
The average Ct for the B fraction and the average Ct for the IN fraction were then used to determine the relative quantity for the target DNA in each fraction by using the equation for the standard curve specific to the primer probe set used.
Relative quantity for qPCR analysis was determined using ΔΔCT method.
The relative quantity for these points was indicated as "not determined" (n.d).
The average relative quantity for each gene under study was calculated and log2-transformed.
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