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This research examines the relative induction of the two primary detoxification pathways upon exposure to nanosilver.
Both resistant (+QTL) cultivars had higher relative induction by M. oryzae infection (black bars) compared to susceptible (-QTL).
This gene was induced at 12 and 24 h in all lines, but the relative induction levels were greater in resistant compared to susceptible cultivars.
Signal intensities for treated samples were background (no cells) subtracted and divided by the intensity of an untreated control to calculate relative induction of apoptosis.
Relative induction of gene mRNA expression was calculated according to the 2−ΔΔCp [28] method and normalized to the expression of 18S.
Relative induction was determined by the 2-ΔΔCt method using GAPDH and the fresh bone marrow extraction for normalization and comparison [22].
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The scan duration (2 min) was chosen because an assessment of the relative heat induction was considered sufficient for a comparison of the two RF transmission settings.
Relative gene induction values were calculated according to the manufacture's instructions.
Thus the relative fold induction of B18R deleted virus by TSA is significantly higher than in WR infected cells (figure 4a).
Delta-delta Ct values of genes were presented as relative fold induction.
Briefly, normalized RT-qPCR tables were loaded in R and relative fold induction was calculated for each cytokine.
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