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At the same time, some relative formulas are modified.
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Thus, the principles of 3D laser scanning and laser triangulation were studied in detail and a relative formula was deduced.
Based on the torsion dynamics theory, the technique and relative formula are presented for the first time for analyzing the actuating voltage and the switch time.
The relative gene expression levels were estimated using the following formula: relative expression = 2−(Ct [target gene] − Ct[ GAPDH ]).
The level of expression was calculated using the formula: Relative expression (t) = (Copy number of target molecule/Copy number of GAPDH) × 1000 [ 14].
Expression levels were evaluated using TaqMan® Gene Expression Assays (Applied Biosystems, Foster City, CA, USA), and GUSB was used as a reference gene for normalization, according to the formula: Relative expression = (Target gene mean quantity/Reference gene mean quantity).
Standard curves of specific CBFs and ACTIN2 were established in every plate to quantify the relative expression levels (the formula of the standard curve quantification method was based on the ABI PRISM 7700 Sequence Detection System user bulletin #2).
Values of expression in fold increase (ratio of control) were calculated using the formula for relative expression by the method of Delta DeltaCT (ΔΔCT): F = 2−ΔΔC.
The normalized mRNA value (NRV) was calculated according to the following formula for relative expression of target mRNA: NRV = (TarS/TarC / ACTB-S/ACTB-C), where TarS/TarC / ACTB-S/ACTB-Cevels of mRNA expression for TarS/TarC / ACTB-S/ACTB-C and control mRNAs, respectively, whereas ACTarS and ACTarC corepresentto amplevels ß- Actin levels in sample and coftrol mRNAs, rexpressiony [ 21].
To transform Ct values to relative expression values, the following formula was used : Relative expression = 2∧ - ΔCt - Ct[stable]).
To determine the relative expression levels, the following formula was used: target gene expression = 2−(Ct[target] − Ct[GAPDH]).
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