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Relative copy number of α2 and α1 gene can be deduced from the ratio of the two peaks.
Relative copy numbers were determined with the ΔΔCt method [69].
Relative Copy Number ("RCN") was calculated as described previously [13].
Three laboratory control DNA samples were used as standards for analyzing relative copy number.
Because these assays are not quantitative, this is not a strict inference of relative copy number.
For quantification of gene relative copy number, we used the comparative Ct method [33].
Relative copy number was calculated by first determining the delta ct (gene of interest minus housekeeping gene) and then using the equation, relative copy number (RCN) = POWER 2,-delta).
The average relative copy number in these regions and for the whole genome was 1.12 and 1.03, respectively.
Δ CTs were converted to relative copy numbers with the formula 2ΔCT (User bulletin 2, Applied Biosystems, http:www.appliedbiosystems.com).
This targeted array specifically assesses relative copy number changes for over 150 human clinically relevant chromosomal loci.
Ancient DNA research generally targets mtDNA because the relative copy number is much higher than their nuclear counterparts.
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